David D. Kline, PhD

  • KLINE_David_2011Professor

Email: KlineDD@missouri.edu

Dalton Cardiovascular Research Center, Room 354

Teaching: Neural Control of the Circulation, Physiology, Neuroscience

Neurohumoral Control of the Cardiovascular and Respiratory System.

Our laboratory focuses on the autonomic nervous system, in particular the cardiovascular and respiratory system. These vital systems operate to keep our bodies within “normal” physiological limits to preserve homeostasis. When challenged acutely or chronically with low environmental oxygen levels (hypoxia) respiration, blood pressure and heart rate compensate to maintain arterial blood gas levels. This can happen during high altitude assent or disease states such as sleep apnea by activation of the chemoreceptor reflex. Additionally, arterial blood pressure is maintained during swings in pressure by the baroreceptor reflex. Both reflex pathways result from activation of neurons in the peripheral and central nervous system. Determining the mechanism of action of these reflex pathways during health and disease is the focus of the laboratory.

Several techniques are used to elucidate these mechanisms. These include 1) radiotelemetry in conscious animals to measure respiration, blood pressure or heart rate; 2) immunohistochemical localization of ion channels and neurotransmitter receptors to specific regions of the nervous system and individual neurons; 3) patch clamp techniques in isolated neurons for recording current flow through ion channels and 4) electrical recording of synaptic transmission in brainstem slices.

Using these techniques, we have recently discovered that chronic intermittent hypoxia, a model for obstructive sleep apnea, elicits a form of neural adaptation or plasticity in the brainstem. This includes changes in neurotransmitter release from presynaptic chemoreceptor afferent neurons as well as postsynaptic action potential firing. We are currently determining the mechanism of this altered neurotransmitter release.

Domingos-Souza G, Martinez D, Sinkler S, Heesch CM, Kline DD (2020) Alpha adrenergic receptor signaling in the hypothalamic paraventricular nucleus is diminished by the chronic intermittent hypoxia model of sleep apnea. Exp Neurol. 2020 Oct 23;335:113517. doi: 10.1016/j.expneurol.2020.113517. Epub ahead of print. PMID: 33132201.

Lima-Silveira L, Martinez D, Hasser EM, Kline DD (2020) Mechanisms Underlying Neuroplasticity in the Nucleus Tractus Solitarii Following Hindlimb Unloading in Rats. Neuroscience. 2020 Nov 21;449:214-227. doi: 10.1016/j.neuroscience.2020.09.043. Epub 2020 Oct 9. PMID: 33039526; PMCID: PMC7679027.

Dantzler HA, Kline DD (2020) Exaggerated potassium current reduction by oxytocin in visceral sensory neurons following chronic intermittent hypoxia. Auton Neurosci. 2020 Dec;229:102735. doi: 10.1016/j.autneu.2020.102735. Epub 2020 Sep 28. Erratum in: Auton Neurosci. 2020 Dec 4;:102758. PMID: 33032244; PMCID: PMC7704630.

Martinez D, Rogers RC, Hasser EM, Hermann GE, Kline DD (2020) Loss of excitatory amino acid transporter restraint following chronic intermittent hypoxia contributes to synaptic alterations in nucleus tractus solitarii. J Neurophysiol. 2020 Jun 1;123(6):2122-2135. doi: 10.1152/jn.00766.2019. Epub 2020 Apr 29. PMID: 32347148; PMCID: PMC7311725.

Matott MP, Hasser EM, Kline DD (2020) Sustained Hypoxia Alters nTS Glutamatergic Signaling and Expression and Function of Excitatory Amino Acid Transporters. Neuroscience. 2020 Feb 4. pii: S0306-4522(20)30057-9. doi: 10.1016/j.neuroscience.2020.01.034. [Epub ahead of print] PubMed PMID: 32032667.

Martinez D, Rogers RC, Hermann GE, Hasser EM, Kline DD (2020) Astrocytic glutamate transporters reduce the neuronal and physiological influence of metabotropic glutamate receptors in nucleus tractus solitarii. Am J Physiol Regul Integr Comp Physiol. 2020 Jan 22. doi: 10.1152/ajpregu.00319.2019. [Epub ahead of print] PubMed PMID: 31967862.

Dalton Cardiovascular Research Center, Room 327-8
– Visit the Kline Laboratory web site